MOTS-cplays lub luag haujlwm tseem ceeb hauv homeostasis ntawm cov metabolism. MOTS-c yog polypeptide uas tsuas muaj 16 amino acids. Kev tshawb fawb qhia tau hais tias MOTS-c kho cov kev cai ntawm mitochondria ntawm insulin los ntawm folate purine AMPK txoj hauv kev. Qhov kev tshawb pom ntawm cov tshuaj hormone no muab lub tswv yim tshiab rau kev kho mob ntshav qab zib, noj zaub mov kom rog thiab lwm yam kab mob. Thiab kev ua haujlwm ntawm cov tshuaj hormones tau raug lees paub ib nrab los ntawm kev sim nas.
Txij li thaum daim ntawv tshaj tawm tau tshaj tawm, kev tshawb fawb txog qhov luv peptide no tau kub heev. Txhawm rau txhawb kev tshawb fawb txog kev tshawb fawb hauv daim teb no, peb lub tuam txhab tau tsim thiab tsim cov khoom lag luam tshawb fawb tag nrho rau MOTS-c.
Ua ntej, peb ua ke MOTS-c peptide (CPX132Hu01) los ntawm cov khoom siv peptide tshiab, thiab nws cov amino acid sequence yog MRWQEMGYIF YPRKLR. Vim yog ib theem zuj zus thiab cov qauv ntawm polypeptide, cov peptide no tuaj yeem siv rau hauv kev sim tsiaj los kawm txog kev ua haujlwm ntawm MOTS-c.
Tom qab ntawd, raws li cov cuab yeej sib txuas me me, cov khoom sib txuas polypeptide tau txuas nrog lub coupling cab kuj kom tau txais tag nrho cov antigen BSA-MOTS-c (CPX132Hu11) thiab OVA-MOTS-c (CPX132Hu21), thiab cov tshuaj tiv thaiv tshwj xeeb (PAX132Hu01) yog tau tom qab txhaj tshuaj tiv thaiv tsiaj nrog tag nrho cov antigen. Cov tshuaj tiv thaiv kab mob no tuaj yeem siv rau ntau yam kev sim molecular thiab kev tiv thaiv kab mob, xws li Western Blot, IHC, thiab lwm yam, txhawm rau txheeb xyuas cov ntsiab lus ntawm MOTS-c hauv cov qauv.
Muag: Edward