1. Taw qhia
Thymosin beta 4 (t 4) Nws yog ib qho me me molecular yuag protein uas muaj cov amino acid residues thiab dav faib nyob rau hauv ntau yam ntaub so ntswg thiab hlwb ntawm tib neeg lub cev. Raws li ib qho ntawm cov actin tswj cov molecules tseem ceeb hauv tib neeg lub cev, nws muaj ntau yam kev ua haujlwm lom neeg thiab ua lub luag haujlwm tseem ceeb hauv cov ntaub so ntswg rov tsim dua tshiab, kho dua tshiab, kho qhov txhab, tswj cov actin tshuav, qog pathogenesis thiab metastasis, apoptosis, o, angiogenesis, kev loj hlob ntawm cov plaub hau follicle. thiab lwm yam physiological thiab pathological txheej txheem.
2.Main Function
T 4 yog ib qho ntawm cov actin tswj cov molecules tseem ceeb hauv tib neeg lub cev, uas muaj ntau yam kev ua haujlwm lom neeg. Nws ua lub luag haujlwm tseem ceeb hauv cov ntaub so ntswg rov tsim dua tshiab, kho dua tshiab, kho qhov txhab, ua kom muaj qhov sib npaug, cov qog pathogenesis thiab metastasis, apoptosis, o, angiogenesis, kev loj hlob ntawm cov plaub hau follicle thiab lwm yam txheej txheem physiological thiab pathological.
3. Daim ntawv thov
Actin regulatory factor actin suav txog li 10 feem pua ntawm tag nrho cov protein nyob rau hauv cov leeg tsis muaj leeg. Nws yog ib qho tseem ceeb ntawm cov qauv ntawm tes, kev txav ntawm tes thiab kho qhov txhab. T hauv hlwb 4 txaus los thaiv tag nrho cov actins, thiab tuaj yeem koom nrog kev tswj hwm ntawm actin polymerization thiab depolymerization. T 4 tuaj yeem ua ke nrog actin monomer hauv qhov sib piv ntawm 1: 1, thiab tiv thaiv kev tsim ntawm F-actin polymer Kev khi ntawm 4 rau actin yog nrog los ntawm kev sib cais ntawm cov dej khi, thiab nws C-terminal khi los ua nws {{ 8}}, ua rau muaj kev hloov pauv ntawm actin monomer. T Muaj ib qho actin binding domain (LKKTET), uas yog lub ntsiab electrostatic tiv tauj qhov chaw. N-terminal fragment ntawm LKKTET tuaj yeem cuam tshuam cov polymerization ntawm actin los ntawm steric hindrance 4 tuaj yeem cuam tshuam cov polymerization ntawm actin thiab kev sib pauv ntawm nucleosides ntawm actin, uas yog qhov tsis sib xws ntawm lwm cov actin binding protein profile, uas tuaj yeem txhawb kev sib pauv ntawm ADP. thiab ATP thiab ceev lub rooj sib txoos ntawm actin 4 thiab profileing tuaj yeem tswj hwm kev sib sau ua ke. T 4 tuaj yeem tswj kev hloov pauv ntawm G-actin thiab F-actin. Nws tau raug tshaj tawm tias nyob rau hauv cov qe ntshav siab heev, t Qhov concentration ntawm T 4 yog 300 pmol Qhov concentration ntawm G-actin yog tsawg dua 20 pmol; Thaum cov concentration nce, t 4 tuaj yeem txo qhov peev xwm depolymerization ntawm F-actin, uas tej zaum yuav yog vim t 4. Nws tuaj yeem tswj kev ua haujlwm ntawm intracellular microfilament system.
4.Quality Standard
Khoom npe | Thymosin 4 Acetate |
Catalog No. | 55820 |
CAS Nr. | 77591-33-4 |
Ib ntus | Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Thr-Glu-Thr-Gln-Glu- Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser-OH |
Molecular Formula | C212H350N56O78S |
Molecular Luj | 4963.49 |
Khaws ntawm | Qhov chaw txias qhuav (-20oC, deb ntawm lub teeb) |
B. SPECIFICATION | |
Kev sim | Specifications |
Qhov tshwm sim | Dawb hmoov |
Purity (HPLC) | >95. 0 feem pua |
Ib qho Impurity (HPLC) | <> |
Amino Acid Composition | 10 feem pua ntawm theoretical |
Cov ntsiab lus Peptide (N%) | >8 0.0 feem pua |
Cov ntsiab lus dej (Karl Fischer) | <> |
Cov ntsiab lus Acetate (HPIC) | <> |
MS (ESI) | Zoo ib yam |
Xam Xaj | 95.0~105.0 feem pua |
Nco tseg: GL Biochem muaj txoj cai los kho cov ntaub ntawv saum toj no yam tsis muaj ntawv tso cai rau txhua tus neeg siv ntawm kev npaj siv cov ntaub ntawv no | |
5.Txoj kev tsom xam
Tom qab cov tshiab los yog khov thymus yog defatted thiab hauv av, ntxiv 3 lub sij hawm tus nqi ntawm ib txwm saline, ua homogenate nyob rau hauv cov ntaub so ntswg crusher, thiab tau txais cov extract (khoom 1) tom qab high-ceev centrifugation. Cov extract tau rhuab ntawm 80 degree rau 15 min kom precipitate lub thermally unstable lwm yam proteins. Tshem tawm cov precipitate los ntawm centrifugation kom tau lub supernatant (khoom 2). Txias lub supernatant mus rau 4 degree, tom qab ntawd ntxiv 5 npaug ntawm qhov ntim ntawm - 10 degree acetone, lim thiab sau cov nag lossis daus, thiab qhuav kom tau acetone hmoov (cov ntsiab lus 3). Dissolve acetone hmoov nyob rau hauv pH7 O nyob rau hauv phosphate buffer tov, ntxiv ammonium sulfate rau saturation ntawm 0 25. Tshem tawm cov nag lossis daus los ntawm centrifugation, kho cov pH ntawm supernatant (khoom 4) mus rau 4. O, thiab tom qab ntawd ntxiv ammonium sulfate rau saturation ntawm 0 50, tau salting tawm. Dissolve cov khoom salted tawm nyob rau hauv phosphate buffer tov nrog pH 8, lim, thiab ces coj lub ultrafiltration nrog molecular hnyav tsawg tshaj li 15000. Lub ultrafiltrate yog desolted los ntawm Sephadex G-25 kem thiab khov-qhuav kom tau thymosin.
6. NMR
7. Kev ruaj ntseg thiab kev nyab xeeb
Kev ruaj ntseg:
Nyob ruaj khov nyob rau hauv cov xwm txheej zoo (chav kub). Cov Ntaub Ntawv Kev ruaj ntseg muaj nyob rau ntawm koj qhov kev thov.
Kev nyab xeeb:
Raws li GARS (Feem ntau Pom Zoo Raws Li Kev Nyab Xeeb) Daim Ntawv Ceeb Toom ntawm Teb Chaws Asmeskas, nws muaj kev nyab xeeb rau tib neeg noj.
8. Flow Chart
9. Cov neeg siv lus pom
Peb muaj cov khw muag khoom ntawm Alibaba, Chemicalbook thiab LookChem, los ntawm cov khoom lag luam zoo thiab cov kev pabcuam tsis tau txais kev pabcuam, peb tau txais ntau cov lus pom zoo.
11.Peb cov neeg muas zaub
Peb tau tsim kev lag luam kev sib raug zoo nrog Abbott, Unilever, Shiseido, KANS thiab SIMM, thiab lwm yam.
12. Exhibitions
Peb feem ntau tuaj koom kev nthuav qhia thoob ntiaj teb, suav nrog CPhI, FIC, API, Vitafoods, SupplesideWest.
Cim npe nrov: thymosin 4 acetate 77591-33-4, manufacturers, lwm tus neeg, Hoobkas, lag luam wholesale, yuav, nqe, zoo tshaj plaws, tej, kev muag khoom
