Human Milk Oligosaccharides HMO

1. Taw qhia

HMO (tib neeg cov mis nyuj oligosaccharides), uas txhais tau tias "tib neeg cov mis nyuj oligosaccharides" los yog "tib neeg cov mis nyuj oligosaccharides", yog peb cov khoom muaj nyob rau hauv tib neeg cov mis nyuj (tsuas yog tom qab rog thiab lactose), nrog rau cov ntsiab lus ntawm 5 ~ 15g / L. , thiab muaj lub luag haujlwm ntawm kev tswj kev tiv thaiv kab mob, pab lub hlwb kev loj hlob thiab tswj txoj hnyuv plab, uas yog qhov zoo rau menyuam mos thiab kev loj hlob

Ntawm ntau tshaj 200 hom oligosaccharide hauv tib neeg cov kua mis, 2 '- fucose (2' - FL) yog HMO ntau tshaj plaws. Cov ntsiab lus thiab hom oligosaccharide hauv cov mis nyuj sib txawv heev ntawm cov mis nyuj hauv tib neeg. Tsuas muaj ntau dua 50 hom oligosaccharide hauv nyuj cov mis nyuj, uas yog qis dua li cov mis nyuj hauv tib neeg. [1] 78 hom oligosaccharide tau pom nyob rau hauv tshis mis nyuj. Cov ntsiab lus ntawm oligosaccharide nyob rau hauv tib neeg cov kua mis yog 5000-20000mg / L, cov ntsiab lus ntawm oligosaccharide nyob rau hauv nyuj cov mis nyuj yog 30-60mg / L, thiab cov ntsiab lus ntawm oligosaccharide nyob rau hauv tshis mis nyuj yog 60-350mg/ L, li 10 npaug ntawm cov mis nyuj. [20]

Hauv cov qauv tshuaj ib txwm siv, tsuas yog prebiotics xws li GOS / FOS yog siv, thaum cov khoom xyaw feem ntau hauv HMO, 2 '- fluorosyllactose (2' - FL), yog ntxiv rau cov mis, uas yog ib qho kev hloov pauv mus rau kev ua tiav ntau qhov tob. simulation ntawm niam mis qauv

2.Main Function

Kev txhim kho thiab kev tiv thaiv kab mob [3]

HMO (2 '- FL) pab tiv thaiv kab mob ntxeem tau thiab txhim kho tus menyuam tus kheej tiv thaiv kab mob [3]

Kev tshawb fawb qhia tias:

70 feem pua ​​​​ntawm tus menyuam lub cev tiv thaiv kab mob muaj nyob hauv cov hnyuv. 2 '- FL tuaj yeem tsim ib qho kev tiv thaiv ntuj tsim nyob rau hauv txoj hnyuv, yog li txo qhov adhesion ntawm Escherichia coli, Salmonella, Helicobacter pylori, thiab lwm yam rau lub cell nto, yog li txo qhov tshwm sim ntawm cov kab mob ntxeem tau [4]

Hauv kev sim vitro, 2 '- FL tuaj yeem cuam tshuam qhov adhesion ntawm E. coli O119 thiab S.fyris [5]

HMO muaj cov tshuaj tiv thaiv kab mob thiab txhawb kev txhim kho thiab tswj kev tiv thaiv kab mob [6]

Lub plasma inflammatory cytokine concentration ntawm cov me nyuam mos noj nrog fortified 2 '- FL mis yog 29 feem pua ​​-83 feem pua ​​qis dua cov me nyuam mos noj nrog mis nyuj tsis tu ncua (p Tsawg dua los yog sib npaug rau 0.05), uas nyob ze rau theem ntawm cov me nyuam mos [7]

Kev tshawb fawb soj ntsuam ua pov thawj tias HMO tuaj yeem pab txo qis qhov tshwm sim ntawm tus kab mob ua pa [8]

Cov txiaj ntsig kev tshawb fawb pom tau hais tias cov niam txiv ntawm cov menyuam mos noj cov mis HMO muaj zog tsawg dua cov neeg mob ntsws ntsws piv rau cov menyuam mos noj mis tsis tu ncua [9]

Piv nrog rau cov menyuam mos noj mis tsis tu ncua, cov menyuam mos uas tau noj cov mis HMO muaj zog muaj tsawg dua cov kab mob ua pa thaum lub sijhawm 0-12 hli, thiab kev siv tshuaj tua kab mob thiab ua npaws txo cov tshuaj txo qis [9]

Txoj kev sim: Cov menyuam mos noj qab nyob zoo hnub nyoog {{{0}}} hnub tau muab faib ua ob pawg: pawg qauv tsis tu ncua (tswj, n=87) thiab pawg HMO fortified (1g/L 2 '- FL thiab 0.5g/L LNnT; xeem, n=88), thiab pub kom txog thaum muaj hnub nyoog 6 hli. Tom qab ntawd, tag nrho cov me nyuam mos tau noj cov mis loj ntawm cov me nyuam mos mus txog 12 lub hlis

3. Daim ntawv thov

HMO prebiotic functions [11]

HMO tuaj yeem tiv thaiv kev zom zaub mov nyob rau sab sauv ntawm cov hnyuv, thiab feem ntau ntawm nws tuaj yeem ncav cuag cov hnyuv me thiab cov hnyuv loj. Nws ua haujlwm raws li lub substrate rau cov kab mob uas muaj txiaj ntsig zoo li Bifidobacterium, txhawb kev loj hlob ntawm probiotics [11]

HMO fermented los ntawm cov kab mob colonic yuav tsim cov lactic acid thiab cov saw luv fatty acid, uas yuav pab kom tus menyuam mos plab hnyuv pH qis, pab txhawb kev loj hlob ntawm probiotics, thiab tseem inhibit qhov kev loj hlob ntawm cov kab mob phem (xws li Escherichia coli thiab Clostridium perfringens) [12 ]

HMO (2 '- FL) txhawb kev loj hlob ntawm Bifidobacterium [13]

Mis mis nyuj oligosaccharide muaj kev tswj xyuas ntawm cov menyuam mos plab hnyuv, ntawm cov uas 2 '- FL ua lub luag haujlwm tseem ceeb hauv kev txhawb nqa kev loj hlob ntawm bifidobacteria [13]

HMO txhawb kev loj hlob ntawm bifidobacteria thiab inhibits kev loj hlob ntawm cov kab mob phem [14]

Tus naj npawb ntawm cov kab mob bifidobacteria muaj txiaj ntsig hauv HMO pawg tau nce siab dua li hauv pawg tswj hwm, thaum tus naj npawb ntawm cov kab mob phem Escherichia thiab Clostridium perfringens qis dua li hauv pawg tswj hwm [15]

Bifidobacterium yog ib qho tseem ceeb microbiota nyob rau hauv lub plab ntawm cov me nyuam mos mos. Kev loj hlob ntawm bifidobacteria yog qhov tseem ceeb rau txoj hnyuv noj qab haus huv ntawm cov menyuam mos thiab cov menyuam yaus [5]

Piv nrog rau cov neeg laus, hom plab hnyuv microbiota hauv cov menyuam mos thiab cov menyuam yaus yog qhov yooj yim, nrog Bifidobacteria yog cov tseem ceeb microbiota hauv plab ntawm cov menyuam mos noj mis.

Hauv cov quav ntawm cov menyuam mos noj qab nyob zoo thiab cov menyuam yaus, bifidobacteria suav txog 80.6 feem pua ​​​​ntawm tag nrho cov kab mob hauv zej zog. Cov kab mob microbial nyob rau hauv cov quav ntawm cov menyuam mos thiab cov menyuam yaus tuaj yeem cuam tshuam txog kev faib tawm ntawm lawv txoj hnyuv microbiota [17]

HMO muaj cov teebmeem prebiotic muaj zog dua FOS [18]

HMO muaj cov teebmeem prebiotic muaj zog dua li qhov sib npaug ntawm FOS, suav nrog kev txhawb nqa kev loj hlob ntawm bifidobacteria, tiam ntawm lactic acid thiab luv saw fatty acids, thiab txo qis pH.

4.Quality Standard

5.Txoj kev tsom xam

Tib neeg cov mis nyuj oligosaccharide (hmo) yog cov khoom tseem ceeb tshaj plaws ntawm tib neeg cov mis nyuj tom qab lactose thiab rog

Qhov thib peb loj tshaj plaws, nrog rau cov ntsiab lus txog li 22-23g / l hauv colostrum thiab 12-13g / l hauv cov mis nyuj laus. Hmo muaj cov kab mob tseem ceeb

Kev kawm ua haujlwm tsis yog tsuas yog muaj kev ua haujlwm ntawm kev tawm tsam plab hnyuv kab mob microbial kab mob, tab sis tseem tswj kev sib npaug ntawm plab hnyuv microbiota.

Siv. Cov mis nyuj muaj pes tsawg leeg yog tus qauv kub rau kev npaj cov me nyuam mos mis hmoov. Yog li, txoj kev tshawb pom ntawm oligosaccharide hauv tib neeg cov kua mis yog tsim

Qhov tsim nyog yuav tsum tau ua ua ntej txhawm rau txheeb xyuas qhov muaj pes tsawg leeg ntawm cov kua mis thiab txhim kho cov mis mos zoo thiab noj qab nyob zoo. Tam sim no, kev tshawb pom ntawm tib neeg cov mis nyuj oligosaccharide

Muaj plaub txoj kev ntsuas tseem ceeb: ion chromatography, kev ua haujlwm siab ua kua chromatography, kua chromatography-mass spectrometry, thiab capillary electrophoresis chromatography. Ntau tog

Cov txheej txheem tshwj xeeb yog raws li hauv qab no: 1) Yog tias cov kua chromatography-mass spectrometry yog nruab nrog lub sijhawm ntawm lub davhlau ntes thiab sib thooj chromatographic kem (porous graphite carbon chromatographic kem)

Ntau) Txog li 300 hom tib neeg cov mis nyuj oligosaccharide tuaj yeem kuaj pom; Txawm li cas los xij, kev tshawb pom ntawm cov kua mis los ntawm cov kua chromatography-mass spectrometry yuav tsum muaj txoj kev kho mob ua ntej.

(tottenetal., ceev-

los ntawm kev siv cov glycomics siv rau tib neeg cov kua mis ligosaccharideprofilingforlargehumanstudies, 2

7935): Centrifuge degreasing with water ->deproteinization with ethanol ->txo nrog nabh4- [graphite carbon nyob ruaj ruaj theem rho tawm: deionization

Water washing desalination ->Elution (20% acetonitrile aqueous solution, 40% Z nitrile and 0.05% trifluoroacetic acid solution)] ->Cia kom qhuav

-Deionized water dissolution ->Ua kua chromatography-mass spectrometry nrhiav kom tau. 2) Yog tias capillary electrophoresis chromatography yog nruab nrog lub ntsuas hluav taws xob fluorescence, nws tuaj yeem sib cais ib txhij

Yog tias UV ntes tau siv los kuaj xyuas cov isomers ntawm ntau tus tib neeg cov kua mis oligosaccharide, tsuas yog qee qhov oligosaccharide tuaj yeem kuaj pom, xws li qaub ncaug.

Acid ntsig txog oligosaccharide. Capillary electrophoresis chromatography rau kev kho mis niam yog raws li nram no (albrecht li al., ce li-

msnprofilingofoligosaccharidesihumanmilkandfecesofbreast-

Fedbibies, 2010, Journal, 31 (issue), 1264-1273): Centrifuge degreasing with water ->deproteinization with Z alcohol ->pob zeb

Ink carbon stationary phase extraction: deionized water washing desalination ->elution (20 feem pua ​​​​acetonitrile aqueous tov, 40 feem pua ​​​​acetonitrile, thiab 0.05 feem pua ​​trifluoride)

Acetic acid solution) - → 9-aminopyrene-1,4,6-triasulfonic acid trisodium salt derivation (overnight) ->Capillary electrophoresis chromatography nrhiav kom tau. 3) Kev siv tus kheej

Tsuas yog qee qhov oligosaccharide hauv tib neeg cov kua mis tuaj yeem kuaj pom los ntawm ion chromatography, kua chromatography thiab huab hwm coj spectrometry.

6.IR: KBr disc

7. Kev ruaj ntseg thiab kev nyab xeeb

Kev ruaj ntseg:

Nyob ruaj khov nyob rau hauv cov xwm txheej zoo (chav kub). Cov Ntaub Ntawv Kev ruaj ntseg muaj nyob rau ntawm koj qhov kev thov.
Kev nyab xeeb:

Raws li GARS (Feem ntau Pom Zoo Raws Li Kev Nyab Xeeb) Daim Ntawv Ceeb Toom ntawm Teb Chaws Asmeskas, nws muaj kev nyab xeeb rau tib neeg noj.

8. Cov neeg siv lus pom

Peb muaj cov khw muag khoom ntawm Alibaba, Chemicalbook thiab LookChem, los ntawm cov khoom lag luam zoo thiab cov kev pabcuam tsis tau txais kev pabcuam, peb tau txais ntau cov lus pom zoo.

9.Peb Cov Ntawv Pov Thawj

Tau ntau xyoo, peb tau cog lus rau cov khoom tsim khoom optimization thiab kev tsim kom zoo. Peb tau teeb tsa qhov kev tswj hwm zoo thiab tau txais daim ntawv pov thawj ntawm nws.

10.Peb cov neeg muas zaub

Peb tau tsim kev lag luam kev sib raug zoo nrog Abbott, Unilever, Shiseido, KANS thiab SIMM, thiab lwm yam.

11. Exhibitions

Peb feem ntau tuaj koom kev nthuav qhia thoob ntiaj teb, suav nrog CPhI, FIC, API, Vitafoods, SupplesideWest.

Cim npe nrov: human milk oligosaccharides hmo, manufacturers, lwm tus neeg, Hoobkas, lag luam wholesale, yuav, nqe, zoo tshaj plaws, tej, kev muag khoom