1. Taw qhiaction

Thiamine pyrophosphate yog coenzyme daim ntawv ntawm Vitamin B1 tam sim no nyob rau hauv ntau cov ntaub so ntswg tsiaj. Thiamine pyrophosphate yog daim ntawv nquag ntawm thiamine, thiab nws ua haujlwm ua cofactor rau ntau cov enzymes koom nrog feem ntau hauv carbohydrate catabolism. Pancreatic cells tau txais thiamin los ntawm lawv ib puag ncig thiab enzymatically hloov nws mus rau hauv thiamin pyrophosphate (TPP) hauv cytoplasm; TPP yog tom qab ntawd coj los ntawm mitochondria los ntawm ib tus neeg nqa khoom tshwj xeeb ntawm mitochondrial TPP transporter (MTPPT; khoom ntawm SLC25A19 noob). Cov khoom qhuav yog ruaj khov thiab cov tshuaj aqueous tsis ruaj khov. Qhov siab tshaj plaws nqus tau tshwm sim ntawm lub wavelength ntawm 242nm ntawm Mp238-240C. Cov khoom no yog soluble hauv dej, pH nqi ntawm aqueous tov yog acidic, tab sis insoluble nyob rau hauv ethanol, acetone thiab ether.ThiaminePyrophosphate, TPP yog ib tug metabolite ntawm vitamin B1 thiab ib cofactor synthesized nyob rau hauv cell solute. Nws yog ib qho tsim nyog los tswj cov kev ua ntawm cell solute transketonase thiab mitochondrial acetone Chemicalbook acid, ketoglutarate thiab branched saw ketoacid dehydrogenase. TPP tuaj yeem siv los ntsuas cov txheej txheem ntawm deacidification hauv cov kab mob lom. TPP tuaj yeem tiv thaiv retinopathy tshwm sim los ntawm hyperglycemia thiab hepatotoxicity tshwm sim los ntawm desflurane hauv nas.

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2. Muaj nuj nqi

2.1 Thiamine pyrophosphate hmoov siv rau vitamin B1 deficiency; vitamin B1 plays lub luag haujlwm tseem ceeb hauv kev tswj cov hlab ntsha ib txwm ua thiab kev ua haujlwm ntawm lub plawv thiab digestive system.

2.2 tuaj yeem siv rau hauv cov txheej txheem decarboxylation hauv cov kab mob lom neeg, thiab tshawb xyuas cov tshuaj tiv thaiv radical ntawm thiamin pyrophosphate hauv 2-oxoacid oxidoreductases.

3.Thiamine pyrophosphate kuj tseem ua lub luag haujlwm tseem ceeb hauv lub paj hlwb, uas tuaj yeem txhawb kev sib txuas thiab tso tawm ntawm cov neurotransmitters thiab tswj lub cev ua haujlwm ntawm lub paj hlwb. Yog li ntawd, thiamine pyrophosphate yog ib qho tseem ceeb heev coenzyme, uas muaj kev cuam tshuam tseem ceeb rau tib neeg noj qab haus huv.

3. Daim ntawv thov

Thiamine pyrophosphate tuaj yeem siv rau kev tshawb fawb biochemical; Nws yog siv rau kev kho mob arrhythmia, myocardial infarction, coma thiab lwm yam kab mob.

Cocarboxylase powder Applications

4. Specifications

Kuaj	Specifications	Kev tshwm sim
Assay	98.0%-101.0%	99.51%
Qhov tshwm sim	Dawb mus rau dawb crystalline hmoov	Ua raws
Loj	100% / 80 mesh	Ua raws
Solubility	soluble hauv dej, thiab pH tus nqi ntawm aqueous kev daws yog acidic. Insoluble hauv ethanol, acetone thiab ether.	Ua raws
daws	Ntshiab rau me ntsis hazy, tsis muaj xim	Ua raws
PH	2.7-3.4	3.0
Tshauv	Tsawg dua lossis sib npaug rau 0.3%	0.16%
Poob rau ziab	Tsawg dua lossis sib npaug rau 5.0%	3.31%
Hnyav hlau	Tsawg dua lossis sib npaug li 20ppm	Tsawg dua lossis sib npaug li 10ppm
Microbiology: Tag Nrho Plate suav Poov & Pwm E.Coli S. Aureus Salmonella	<1000cfu/g <100cfu/g Negative Negative Tsis zoo	80cfu/g 50cfu/g Ua raws Ua raws
Xaus	Raws li Standard

5.Flow chart

6. NMR

Cocarboxylase NMR

7. Kev ruaj ntseg thiab kev nyab xeeb

Peb tau ua ntau yam kev ruaj ntseg thiab kev nyab xeeb kawm txog cov khoom no, raws li cov ntaub ntawv ceev ceev thiab kev ruaj ntseg mus sij hawm ntev, nws qhia cov yam ntxwv ruaj khov; Raws li kev tshawb nrhiav tsiaj txhu, nws qhia tau tias muaj kev nyab xeeb.

8. Kev xeem txoj kev

I. ntsuas thiab ntsuas tshuaj.
Kev ua haujlwm siab ua kua chromatography nrog ultraviolet ntes (Shimadzu); hluav taws xob tshuav nyiaj li cas.
Thiamine pyrophosphate siv tshuaj, methanol thiab acetonitrile li chromatographic purity, sodium heptane sulfonate ua tshwj xeeb reagent rau kua chromatography.
2. Cov txheej txheem.
2.1. Chromatographic tej yam kev mob.
Chromatography kab: Inertustain C18 (5 μm, 4.6mm * 250mm).
Mobile theem: methanol-acetonitrile-0.02mol/L heptanesulfonate sodium tov (muaj 1% triethylamine, kho pH rau 5.5 nrog phosphoric acid) (7-7-14-8-6-5-methanesulfonate).
Tus nqi ntws: 1.0ml / min; nrhiav wavelength: 246nm; sab kub: 30 degree; txhaj tshuaj ntim: 20μL.
2.2 Kev npaj cov kev daws teeb meem.
Cov qauv tswj hwm ntawm thiamine pyrophosphate, txog 25mg, yog qhov hnyav thiab muab tso rau hauv 25ml xim av lub raj mis thiab diluted mus rau qhov ntsuas los ntawm lub sijhawm txawb, uas tau siv los ua cov ntaub ntawv pov thawj cov tshuaj (1mg / ml). Precision 5ml raug coj mus rau diluted rau scale nyob rau hauv 100ml xim av lub raj mis, uas yog siv raws li siv tshuaj (50 μ g / ml).
2.3 Kev npaj cov qauv kev daws teeb meem.
Ntsuas tus qauv 50mg precisely, muab tso rau hauv 50ml xim av ntsuas lub raj mis thiab dilute nws mus rau lub teev nrog mobile theem. 5ml ntawm cov tshuaj saum toj no tau muab coj los ua kom raug thiab diluted mus rau qhov ntsuas hauv 100ml xim av lub raj mis, thiab lub sijhawm txawb tau siv los ua cov qauv tshuaj (50 μ g / ml).
3. Kev txiav txim siab.
Qhov tsim nyog ntawm cov tshuaj tiv thaiv cov tshuaj tiv thaiv (1mg / ml) tau muab coj los ua thiab diluted mus rau cov kev siv tshuaj ntawm 1,10,20,50,100,200 μg / ml nrog rau theem mobile feem. Raws li cov xwm txheej saum toj no chromatographic, 20 μ L tau raug nqus thiab txhaj rau hauv cov kua chromatograph, thiab cov chromatogram raug kaw. Siv cov concentration (μ g / ml) raws li Abscissa thiab thaj chaw ncov raws li kev tswj hwm, linear regression tau ua thiab tau txais qhov sib npaug regression.
Cov qauv tshuaj ntsuam xyuas tau npaj raws li cov qauv saum toj no, 20 μ L ntawm cov qauv tshuaj tau nqus thiab txhaj rau hauv cov kua chromatograph, thiab cov ntsiab lus ntawm thiamine pyrophosphate raug xam raws li cov xwm txheej saum toj no chromatographic.

9. Daim ntawv pov thawj

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