CAS 13870-90-1 Cobamamide Powder

1.Introkev txiav txim siab

Cobamamide hmoov yog ib qho ntawm cov khoom lag luam ntawm cov vitamin B12, uas yog lub npe hu ua 5-dimethylbenzimidazole-5-deoxyadenine nucleoside cobalamin, uas yog octahedral cobalamin complex. Nws yog ib qho tsaus liab crystalline thiab tsis-crystalline hmoov nrog muaj zog hygroscopicity thiab yooj yim rau decompose nyob rau hauv lub teeb. Nws yog ib qho tseem ceeb ntawm cov vitamin B12 series cov khoom lag luam, uas yog cov khoom siv raw khoom thiab muaj cov nqi kho mob tseem ceeb.

Nws yog ib qho analogue ntawm cyanocobalt vitamin B12, uas yog ib qho tseem ceeb coenzyme rau nucleotide synthesis nyob rau hauv hlwb, koom nyob rau hauv methyl conversion thiab folic acid metabolism, txhawb kev txo cov methyl folic acid rau tetrahydrofolic acid, thiab kuj koom nyob rau hauv lub tricarboxylic acid voj voog, uas yog ib qho tseem ceeb heev rau Chemicalbook tsim ntawm lipoproteins nyob rau hauv lub myelin sheath, uas yuav ua rau sulfhydrylase qhuav active, yog li koom nyob rau hauv ib tug ntau yam ntawm cov protein thiab rog metabolism. Cov khoom no tuaj yeem txhawb txoj kev loj hlob thiab kev loj hlob ntawm cov qe ntshav liab, uas yog qhov tsim nyog rau kev ua tiav ntawm cov paj hlwb txha caj qaum fibers thiab tswj kev ua haujlwm ntawm cov hlwb epithelial ntawm lub plab zom mov.

2. Muaj nuj nqi

2.1 Cobamamide hmoov yog siv rau cov kab mob tshwm sim los ntawm vitamin B12 tsis txaus, xws li anemia thaum cev xeeb tub thiab tsis muaj zaub mov noj. Nws kuj tuaj yeem siv rau hauv kev kho mob ntawm cov kab mob noj zaub mov thiab leukopenia tshwm sim los ntawm hluav taws xob thiab tshuaj.

Vitamin B-12 concentrations nyob rau hauv cov kua mis ntawm 68 vitamin B-12-cov niam ntxiv tau ntau dua li ntawm 73 tus poj niam uas tau muab cov placebo thaum supplementation xaus ntawm 6 wk postpartum (Table 2). Txawm li cas los xij, cov kua mis vitamin B-12 concentrations ntawm 3 thiab 6 lub hlis tom qab yug me nyuam tsis txawv ntawm cov pab pawg kho mob.

2.2 Kev tshawb fawb tshuaj thiab cov ntaub so ntswg kab lis kev cai. Cov tshuaj kho mob yog cov vitamin B pawg, uas koom nrog hauv kev sib txuas ntawm nucleic acid, choline, methionine thiab cov metabolism hauv rog thiab qab zib hauv vivo. Lawv siv los kho ntau yam megaloblastic anemia, malnutrition anemia thiab lwm yam kab mob.

3. Daim ntawv thov

Cobamamide hmoov siv rau kev lag luam kws tshuaj.

4. Specifications

5.Flow chart

6. Kev xeem

QHIA

• KEV PAB CUAM

Kev daws A: Methanol

Tshuaj B: Dissolve 6.8 g ntawm monobasic poov tshuaj phosphate hauv 950 mL dej. Kho nrog 10 feem pua ​​​​phosphoric acid rau pH ntawm 3.2.

Dilute nrog dej mus rau 1000 mL. Dhau qhov kev daws teeb meem no los ntawm ¦lter ntawm 0.2-µm pore loj.

Mobile theem: Saib Table 1.

Diluent: Methanol thiab dej (1: 3)

[CEEB TOOM—Siv cov iav uas tsis tshua muaj actinic, thiab tiv thaiv cov kev daws teeb meem hauv qab no los ntawm kev raug lub teeb.]

Kev daws teeb meem tsim nyog: 0.05 mg/mL txhua ntawm USP Cobamamide RS, USP Cyanocobalamin (Crystalline) RS, USP Hydroxocobalamin

Chloride RS, thiab USP Methylcobalamin RS hauv Diluent

Kev daws teeb meem: Tso cai rau ib feem ntawm USP Cobamamide RS kom sib npaug ntawm chav tsev txheeb ze thiab tiv thaiv

los ntawm lub teeb, kom txog rau thaum nws qhov hnyav qhia tsis muaj kev hloov pauv. Lub sij hawm sib npaug yuav ntev txog 60 min lossis ntau dua nyob ntawm tus txheeb ze ntawm chav tsev

av noo. Siv cov khoom sib npaug los npaj cov tshuaj 1-mg/mL hauv Diluent. Concurrently txiav txim siab cov ntsiab lus dej ntawm lub

equilibrated qauv thiab siv nws rau Assay kho.

Cov qauv kev daws teeb meem: Npaj raws li cov lus qhia hauv Standard tov, tshwj tsis yog hloov USP Cobamamide RS nrog Cobamamide.

Chromatographic system

(Saib Chromatography 〈621〉, System Suitability.)

Qauv: LC

Detector: UV 260 nm

Kem: 4.6-mm × 10-cm; 2.6-µm ntim L1

Kem kub: 40 degree

Tus nqi ntws: 1.2 mL / min

Txhaj ntim: 5 µL

Qhov system haum

Qauv: Cov kev daws teeb meem tsim nyog thiab kev daws teeb meem

[CEEB TOOM—Lub sij hawm tuav tus txheeb ze rau cobamamide, hydroxocobalamin, cyanocobalamin, thiab methylcobalamin yog 1.00, 0.15, 0.40,

thiab 1.47, ntsig txog, System suitability solution.]

Suitability xav tau

Kem e¨ciency: NLT 22, 000 theoretical plates for the cobamamide ncov, Standard solution

Tailing yam: NMT 2.0 rau lub ncov cobamamide, Standard tov

Kev tsom xam

Cov Qauv: Cov qauv kev daws teeb meem thiab cov qauv kev daws teeb meem

Xam cov feem pua ​​​​ntawm cobamamide (C72H100CoN18O17P) hauv feem ntawm Cobamamide noj:

Cov txiaj ntsig=(rU/rS) × (CS/CU) × 100

rU= cov lus teb siab tshaj ntawm cobamamide los ntawm cov qauv tshuaj

rS=cov lus teb siab tshaj ntawm cobamamide los ntawm Standard tov

CS=concentration ntawm USP Cobamamide RS (kho rau cov ntsiab lus dej) hauv Standard tov (mg/mL)

CU=concentration ntawm Cobamamide (kho rau cov ntsiab lus dej) hauv cov tshuaj Sample (mg/mL)

Cov qauv lees txais: 96.0 feem pua ​​-102.0 feem pua ​​​​ntawm cov dej tsis muaj dej

7. Kev ruaj ntseg thiab kev nyab xeeb

Peb tau ua ntau yam kev ruaj ntseg thiab kev nyab xeeb kawm txog cov khoom no, raws li cov ntaub ntawv ceev ceev thiab kev ruaj ntseg mus sij hawm ntev, nws qhia cov yam ntxwv ruaj khov; raws li kev tshawb nrhiav tsiaj txhu, nws qhia tau tias muaj kev nyab xeeb.

8. Daim ntawv pov thawj

9. Exhibition

10.Peb Cov Neeg Siv Khoom

11.Cov neeg siv tshuaj xyuas

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