Bilirubin hmoov CAS 635-65-4

1. Taw qhia

Bilirubin hmoov CAS 635-65-4 yog cov saw zoo li tetrapyrrole compound txuas los ntawm ob lub methylene txuas hniav thiab ib tus choj methylene, muaj plaub lub nplhaib pyrrole. Txawm hais tias bilirubin muaj cov hydrophilic pawg xws li 2 hydroxyl lossis ketone pawg, 4 pawg amino, thiab 2 pawg carboxyl, thiab nws cov qauv zoo ib yam li bilirubin, uas kuj yog cov xim bilirubin, nws solubility hauv dej yog qhov phem dua li biliverdin. Yog vim li cas rau qhov tshwm sim no yog tias pawg hydrophilic ntawm bilirubin tsim rau rau intramolecular hydrogen bonds nyob rau hauv lub molecule, dov tag nrho cov molecule rau hauv ib tug nruj folding qauv nyob rau hauv cov duab ntawm tus txha nraub qaum. Cov pawg polar tau muab zais rau hauv cov molecule thiab tsis tuaj yeem tsim hydrogen bonds nrog dej molecules thiab yaj hauv dej. Yog li ntawd, bilirubin yog ib yam khoom uas tsis yog-polar lipid soluble uas nyuaj rau yaj hauv dej, tab sis muaj kev sib raug zoo rau plasma albumin.

2. Muaj nuj nqi

2.1 Lub hauv paus tseem ceeb yog txo cov khoom ntawm heme hauv hemoglobin tom qab kev tawg ntawm cov qe ntshav liab. Nws yog ib tug monoclinic siv lead ua nrog ib tug golden los yog tob reddish xim av, tsw thiab tasteless. Soluble hauv cov kuab tshuaj organic xws li benzene, chloroform, thiab carbon disulfide; Nws tuaj yeem yaj hauv qhov sib xyaw ntawm ethanol kub thiab chloroform; Sodium ntsev yog soluble hauv dej, tab sis calcium ntsev, magnesium ntsev, thiab barium ntsev yog insoluble hauv dej. Cov khib nyiab qhuav yog qhov ruaj khov; Chloroform tov kuj tseem ruaj khov nyob rau hauv qhov tsaus ntuj; Hauv cov tshuaj alkaline (xws li 0.1mmol/L sodium hydroxide) los yog thaum ntsib trivalent hlau ions, nws tsis ruaj khov thiab sai oxidized rau biliverdin. biliverdin tuaj yeem txo qis rau bilirubin los ntawm cov tshuaj lom neeg hauv vitro lossis biliverdin reductase. Bilirubin tuaj yeem khi rau glycine, alanine, lossis histidine. Bilirubin hmoov CAS 635-65-4 yog ib qho muaj zog endogenous antioxidant ntawm cov ntshav ntshav ntau. Cov qauv molecular ntawm bilirubin tuaj yeem muaj ob hom: ketone thiab cawv. Vim yog cov qauv coiled ntawm bilirubin, nws cov hydrophobicity nce thiab nws tuaj yeem nkag mus rau sab hauv ntawm lipid molecules. Bilirubin muaj qhov txuas txuas txuas ntxiv ob qhov nyiaj sib txuas thiab cov khoom siv hydrogen atoms, uas tuaj yeem tiv thaiv oxidation. Qhov kev sim qhia tau hais tias bilirubin tuaj yeem cuam tshuam kev hloov pauv oxidative ntawm LDL (tsis tshua muaj lipoprotein nyob rau hauv cov ntshav) rau qee yam hauv vitro, uas tuaj yeem cuam tshuam thiab txo qis kev tsim cov atherosclerosis los ntawm oxidative hloov pauv ntawm LDL, thiaj li ua tiav lub hom phiaj ntawm kev ua haujlwm ntawm lub cev. tiv thaiv thiab kho kab mob plawv.

2.2 Lub hauv paus tseem ceeb yog txo cov khoom ntawm heme hauv hemoglobin tom qab kev tawg ntawm cov qe ntshav liab. Nws yog ib tug monoclinic siv lead ua nrog ib tug golden los yog tob reddish xim av, tsw thiab tasteless. Soluble hauv cov kuab tshuaj organic xws li benzene, chloroform, thiab carbon disulfide; Nws tuaj yeem yaj hauv qhov sib xyaw ntawm ethanol kub thiab chloroform; Sodium ntsev yog soluble hauv dej, tab sis calcium ntsev, magnesium ntsev, thiab barium ntsev yog insoluble hauv dej. Cov khib nyiab qhuav yog qhov ruaj khov; Chloroform tov kuj tseem ruaj khov nyob rau hauv qhov tsaus ntuj; Hauv cov tshuaj alkaline (xws li 0.1mmol/L sodium hydroxide) los yog thaum ntsib trivalent hlau ions, nws tsis ruaj khov thiab sai oxidized rau biliverdin. biliverdin tuaj yeem txo qis rau bilirubin los ntawm cov tshuaj lom neeg hauv vitro lossis biliverdin reductase. Bilirubin tuaj yeem khi rau glycine, alanine, lossis histidine. Bilirubin yog ib qho muaj zog endogenous antioxidant ntawm cov ntshav ntshav ib txwm muaj. Cov qauv molecular ntawm Bilirubin hmoov CAS 635-65-4 tuaj yeem muaj ob hom: ketone thiab cawv. Vim yog cov qauv coiled ntawm bilirubin, nws cov hydrophobicity nce thiab nws tuaj yeem nkag mus rau sab hauv ntawm lipid molecules. Bilirubin muaj qhov txuas txuas txuas ntxiv ob qhov nyiaj sib txuas thiab cov khoom siv hydrogen atoms, uas tuaj yeem tiv thaiv oxidation. Qhov kev sim qhia tau hais tias bilirubin tuaj yeem cuam tshuam kev hloov pauv oxidative ntawm LDL (tsis tshua muaj lipoprotein nyob rau hauv cov ntshav) rau qee yam hauv vitro, uas tuaj yeem cuam tshuam thiab txo qis kev tsim cov atherosclerosis los ntawm oxidative hloov pauv ntawm LDL, thiaj li ua tiav lub hom phiaj ntawm kev ua haujlwm ntawm lub cev. tiv thaiv thiab kho kab mob plawv.

3. Daim ntawv thov

3.1 Bilirubin hmoov CAS 635-65-4 yog ib qho tseem ceeb ntawm heme decomposition thiab cov xim tseem ceeb hauv cov kua tsib. Nws ua hauj lwm raws li ib tug antioxidant thiab zoo hydrogen peroxide scavenger, tiv thaiv cell membrane lipids los ntawm oxidative los ntawm cov reactive pawg.
3.2 Feem ntau yog siv rau kev tshawb fawb biochemical, analytical chemistry, thiab synthesis ntawm bezoar. Nws kuj tseem tuaj yeem siv los ua antioxidant los tiv thaiv daim nyias nyias phospholipids los ntawm oxidation los ntawm hydrogen peroxide dawb radicals, thiab tuaj yeem tiv thaiv cov neurons los ntawm oxidative puas tsuaj txawm nyob rau ntawm nanomolar concentrations.
3.3 Bilirubin hmoov CAS 635-65-4 kuj tseem siv rau kev txiav txim siab ntawm cov khoom siv bovine bilirubin. Bilirubin muaj ntau yam tshuaj muaj txiaj ntsig thiab yog cov khoom siv tseem ceeb rau kev tsim cov bezoar.
Nws muaj cov nyhuv inhibitory zoo ntawm W256 qog, thiab nws qhov inactivation rate thiab inhibition Performance index tiv thaiv tus kab mob Japanese encephalitis yog 1-1.5 npaug ntau dua li deoxycholic acid thiab cholic acid; Nws kuj yog ib qho tshuaj kho tau zoo rau cov kab mob siab, nrog rau kev muaj peev xwm nthuav tawm cov hlwb tshiab yam tsis muaj kev puas tsuaj rau daim siab, thiab tuaj yeem kho cov kab mob xws li ntshav siab thiab mob cirrhosis,
Tsis tas li ntawd, bilirubin muaj sedative, sedative, antipyretic, thiab antihypertensive zog. Txhawb txoj kev tsim cov qe ntshav liab thiab lwm yam haujlwm.

4.Quality Standard

5. Kev tsim nyog

Tom qab xyoo ntawm kev tsim khoom, peb lub tuam txhab tau txais daim ntawv tso cai tsim khoom tsim nyog.

6 Txoj kev kuaj xyuas

6.1 Chromatographic kem Hypasi Kub C18(4.6mm ✖ 250mm) Ultra-ntshiab silica gel kem;

Mobile theem:

D: Glacial acetic acid tov

C: Acetonitrile

C: D =95:5;

Kem kub: 30 degree;

Ntsuas wavelength: 450nm;

Txhaj ntim: 5 μl. koob ntxuav thiab txhaj tshuaj;

Pump flow rate: 1.0ml/min

Nres sijhawm: 13 min

6.2 Kev txiav txim siab
Raws li cov saum toj no chromatographic kev khiav hauj lwm tej yam kev mob, tom qab lub ntsuas ntsuas yog ruaj khov, txhaj ob peb txhaj tshuaj ntawm cov txheej txheem kev daws teeb meem mus txog rau thaum lub ncov dav hlau uas nyob ib sab ob txhaj tshuaj mus txog.
Tom qab qhov kev hloov pauv hauv cov lus teb muaj nqis tsawg dua 1.0%, kev ntsuas yuav tsum tau ua raws li cov qauv kev daws teeb meem, cov qauv kev daws teeb meem, cov qauv kev daws teeb meem, thiab cov qauv kev daws teeb meem.
6.3 Qhov nruab nrab ntawm qhov chaw siab tshaj ntawm bilirubin hauv ob txoj kev daws teeb meem thiab ob txoj kev daws teeb meem ua ntej thiab tom qab tus qauv.

Hauv cov qauv:
A1-----Qhov nruab nrab qhov siab tshaj plaws ntawm bilirubin hauv cov qauv kev daws teeb meem;
A2----Qhov nruab nrab qhov siab tshaj plaws ntawm bilirubin hauv cov qauv tshuaj:
m1---Tus nqi raug ntawm tus qauv loj ntawm tus qauv bilirubin, ntsuas hauv milligrams (mg):
m2--- Tus nqi raug ntawm tus qauv loj ntawm bilirubin, ntsuas hauv milligrams (mg);
P----Qhov loj feem ntawm P-bilirubin tus qauv,%.
Siv tus lej lej ntawm ob qhov kev ntsuas qhov sib npaug raws li qhov ntsuas tau tshaj tawm, thiab qhov sib txawv ntawm ob qhov kev ntsuas qhov sib npaug yuav tsum tsis pub tshaj 1.5%.

7 HPLC kuaj pom chromatogram

8 Flow Chart

Kev npaj cov tshuaj hydrolysis los ntawm ion txauv resin extraction method:
8.1 Noj cov kua tsib tshiab, lim nws nrog gauze, ntxiv 2mol / L sodium hydroxide tov los kho cov pH rau 10 thaum nplawm, boil thiab lim kom tau cov hydrolysate. 8.2 Adsorption thiab elution ntawm bile acid los ntawm hydrolysis tov resin kem: ncuav boiled hydrolysis tov rau hauv preheated resin kem kom txog rau thaum cov xim ntawm cov effluent darker thiab cov adsorption yog saturated.
8.3 Ntxiv dej txias kom txog rau thaum qhov kub hauv kem poob rau hauv chav tsev kub, thiab tom qab ntawd ntxiv cov tshuaj sodium bisulfite hydrochloride dilute los ntawm sab saum toj ntawm kem kom cov pH ntawm cov effluent 1.
8.4 Ntxuav nrog 75% ethanol, sau thaum lub eluent yog ib tug tsaus ntsuab turbid tov, thiab ntxuav kom txog thaum lub effluent yuav ntshiab thiab pH nyob ze nruab nrab. Ntxuav dua nrog 95% ethanol.
8.5 Sau cov tshuaj ethanol elution rau kev npaj cov kua tsib kua qaub. Hydrolysis tov [ion exchange kem] → [75% ethanol, 95% ethanol] Ethanol eluent rau bilirubin elution, concentration, thiab ziab: Tso tag nrho 95% ethanol los ntawm kem, soak hauv chloroform rau 10 feeb, thiab sau chloroform eluent kom txog thaum tsis muaj xim daj tshwm.
8.6 Distille cov chloroform eluent kom ze rau qhov qhuav, ntxiv anhydrous ethanol, thiab txuas ntxiv mus kom txog thaum tsis muaj chloroform. Lim thaum kub, sau bilirubin, qhuav, thiab tau cov khoom tiav bilirubin.

9.Patent cov ntsiab lus

Tom qab ntau xyoo ntawm kev ua haujlwm nyuaj, Kono Chem Co., Ltd tau txais ntau cov patented technologies.

Hauv qab no yog tus lej patent:

CN113527166A

CN112934445A

CN213006706U

CN212731128U

CN212576285U

CN212467595U

CN212467207U

PIB CN112961024A

10. Tus khub

Peb muaj ntau tus neeg koom tes mus sij hawm ntev thoob plaws ntiaj teb.

11.Laboratory thiab Hoobkas ib puag ncig

12. Exhibitions

Peb lub tuam txhab koom nrog ntau yam kev nthuav qhia hauv tsev thiab txawv teb chaws txhua xyoo, thiab sib txuas lus nrog cov neeg siv khoom ntsej muag.

Cim npe nrov: bilirubin hmoov cas 635-65-4, Tuam Tshoj bilirubin hmoov cas 635-65-4 manufacturers, lwm tus neeg, Hoobkas