Rotenone hmoov

1 Kev Taw Qhia

Rotenone yog ib qho tshuaj organic nrog cov mis mos Molecular C23H22O6. Nws muaj nyob rau hauv cov hauv paus hniav ntawm pistaceae, pistaceae thiab rattan nroj tsuag nyob rau hauv lub tropical thiab subtropical cheeb tsam ntawm Asia, thiab kuj nyob rau hauv cov hauv paus hniav ntawm Suav tshuaj ntsuab, xws li Diguazi, Kutanzi, thiab Kunming Spatholobus. Yuav luag insoluble hauv dej, soluble hauv ethanol, acetone, carbon tetrachloride, chloroform, ether, thiab ntau lwm yam organic solvents. Thaum raug lub teeb thiab huab cua, nws decomposes. Nws cov tshuaj hauv cov kuab tshuaj organic tsis muaj xim. Thaum nws raug huab cua, nws yuav raug oxidized rau daj, txiv kab ntxwv thiab tom qab ntawd sib sib zog nqus liab, thiab muaj peev xwm precipitate dehydrogenated Rotenone thiab rotenone crystals uas yog lom rau kab.

2 Muaj nuj nqi

Rotenone, ib yam khoom uas muaj zog tshwj xeeb hauv toxicology, dav muaj nyob rau hauv cov cag ntoo ntawm cov nroj tsuag, thiab muaj kev sib cuag thiab plab toxicity cuam tshuam rau kab, tshwj xeeb tshaj yog Pieris rapae larvae, Diamondback npauj thiab aphids. Cov kev tshawb fawb thaum ntxov tau pom tias Rotenone cov txheej txheem ntawm kev ua haujlwm yog feem ntau cuam tshuam rau Cellular respiration ntawm kab, feem ntau nrog ib feem ntawm NADH dehydrogenase thiab coenzyme Q. Rotenone suppresses Electron thauj saw ntawm kab kab, yog li txo qis ATP theem hauv kab mob thiab thaum kawg. ua rau kab tsis tuaj yeem tau txais lub zog, thiab tom qab ntawd ua haujlwm maj mam, tuag tes tuag taw thiab maj mam tuag.

Mitochondria, NADH dehydrogenase, succinic acid, Mannitol thiab lwm yam tshuaj nyob rau hauv ntau lub hlwb yog rhiab heev rau Rotenone. Kev hloov hluav taws xob los ntawm NADPH mus rau NADH hauv Setayria cervi mitochondria tuaj yeem cuam tshuam los ntawm Rotenone, thiab kev ua haujlwm ntawm fumaric acid reductase system hauv Setaria digitata mitochondrial granules yog rhiab rau rotenone. Rotenone thiab salicylhydroxamic acid tuaj yeem cuam tshuam EMT ntawm Trypanosoma brucei Inner mitochondrial membrane, yog li cuam tshuam ncaj qha rau kev ua ntawm NADH dehydrogenase; Rotenone kuj tseem tuaj yeem cuam tshuam cov haujlwm ntawm NADH rau cytochrome C thiab NADH rau coenzyme Q reductase hauv cov hlab ntsws ntawm Trypanosoma brucei brucei mitochondria, nrog rau inhibition ntawm 80 feem pua ​​~ 90 feem pua.

3 Daim ntawv thov

Aphids, planthoppers, striped fleas, thrips, daj mugworts, ape leafworms, cabbage caterpillars, spodoptera litura, beet Armyworm, Diamondback npauj, thiab lwm yam.

Txhawm rau tswj cov kab ntsig kab ntsig thiab aphids, 80 ~ 120ml ntawm 4 feem pua ​​Rotenone emulsifiable concentrates ib mu thiab 30kg tsuag nrog dej.

Txhawm rau tswj cov npauj Diamondback, tshuaj tsuag 80 ~ 160ml ntawm 4 feem pua ​​Rotenone emulsifiable concentrates nrog 30kg dej hauv tshuaj tsuag.

Rau kev tswj hwm ntawm Spodoptera litura, kev npaj ntawm 4 feem pua ​​Rotenone emulsifiable concentrates (80-120ml) yog sprayed nrog 30kg dej (txhaj tshuaj) ib mu.

Txhawm rau tiv thaiv thiab tswj cov kab tsuag zaub, kev npaj ntawm 4 feem pua ​​​​Rotenone emulsifiable concentrates (80-160ml) yog txau nrog 30kg dej (txhaj tshuaj) ib mu.

1. Muaj zog tshwj xeeb thiab ceev cov kab tsuag; Nws muaj zog tua cov kab, tshwj xeeb tshaj yog aphids, thrips thiab diving beetles, thiab muaj zog tiv thaiv tua thiab plab lom nyhuv ntawm Pieris rapae larvae, Diamondback npauj thiab lwm yam larvae.

2. Lub neej ntev; Cov tshuaj tua kab ntawm Rotenone ntev, txog 10 hnub.

3. Yooj yim rau decompose thaum raug lub teeb, nrog tsawg tsawg residue; Cov kua tshuaj nyob rau saum npoo ntawm nplooj yog yooj yim decomposed thaum raug lub teeb thiab tsis ua phem rau ib puag ncig. Lub sijhawm thov yog 3 hnub.

4. Rau ib puag ncig thiab tsiaj kev nyab xeeb. Rotenone tsis yog tsuas yog tsim kev puas tsuaj rau Aquatic tsiaj, tab sis kuj muaj kev nyab xeeb rau tib neeg thiab tsiaj txhu thiab yuav tsis ua phem rau ib puag ncig.

Kev tiv thaiv thiab tswj lub hom phiaj: aphids, daj kab txaij fleas, thrips, daj melon, ape nplooj kab, cabbage beetles, thiab lwm yam.

1. Siv 4 feem pua ​​Rotenone emulsifiable concentrate los ntxuav lub pas dej rau cov cw kab lis kev cai: siv 0.025mg/L Rotenone rau tshuaj lom ntses, tab sis cws tuaj yeem muaj sia nyob hauv 10mg/L Rotenone tov. Rotenone yog kev puas tsuaj rau ntses thiab lwm yam kab mob hauv thaj chaw loj, yog li nws tsim nyog rau kev cog qoob loo thiab lwm cov cw kom tshem cov pas dej. Feem ntau, 667m2 dej nto thiab 1m dej tob yog siv, thiab 4 feem pua ​​Rotenone emulsifiable concentrate 1000ml tuaj yeem siv los ntxuav cov pas dej. Rotenone kuj tuaj yeem siv los ua Molluscicide thiab ntes ntses Aquarium.

2. Catching ornamental ntses: Ntau hom ntses yuav sai sai no nyob rau hauv lub lethal concentration ntawm Rotenone tov ({1}}.025mg/L). Lub sijhawm no, yog tias lawv hloov mus rau 5-10mg / L concentration ntawm methylene ntsev tov nyob rau lub sijhawm, cov ntses no yuav rov qab los sai sai. Siv cov yam ntxwv ntawm Rotenone, cov ntses Aquarium zoo tuaj yeem ntes tau. Txoj kev no feem ntau yog siv rau hauv qee qhov kev tshawb fawb thiab kev tshawb fawb.

3. 5-7 feem pua ​​Fish Vine Root Powder Cleaning Pond: 6-8 grams paus hmoov ib cubic meter ntawm 667 square metres ntawm dej saum npoo qhov tob.

4 Tus Qauv Zoo

5 Flow Chart

Sodium azide ~ monomethylamine ~ Carbon disulfide ~ Ethyl chloroformate

6 NMR

7.MOA

1.1 Cov cuab yeej thiab cov tshuaj reagents

Instrument: ARIS Q-hom GO/MS gas chromatography-mass spectrometry

Khoom siv; Khoom siv: Rotenone standard (Sigma Company, USA, purity

99 feem pua); Reagents: chloroform, methanol, dej deionized, thiab lwm yam.

1.2 Chromatographic tej yam kev mob

GC condition: HP{{0}}MS (30m × 0.25ram × 0.259m);

Kem kub 200 degree (2 feeb) 40 degree / min 280 degree (16 feeb); Txhaj chaw nres nkoj

28 0 degree; Carrier gas: Helium gas, tas li ntws 1.0ml / min, phua piv 20: 1.

MS mob: EI ion qhov chaw; ionization voltage 70ev; Txoj kev scanning:

Luam theej duab; Kev soj ntsuam zoo ntau yam: 40-450amu; ion qhov kub ntawm 220 degree;

Transmission kab kub 2800C; Lwm yam xwm txheej yog tus nqi Auto-Tune.

1.3 Kev npaj ua qauv

Nqa cov rattan hauv paus thiab qia ntawm cov plaub hau ntses txog 29, txiav rau hauv pieces raws txoj kev loj hlob, thiab muab tso rau lawv.

Tom qab ntxuav ultrasonic hauv dej deionized, cua qhuav thiab muab tso rau hauv qhov cub cua tshuab ziab khaub ncaws ntawm 60 degree

Txo kom qhuav. Soak cov qauv npaj hauv 5mL chloroform tov rau kwv yees li

Tom qab 15 feeb, centrifuge ntawm kev kub ceev thiab nqa cov kua ntshiab los ntawm 0.45 tma organic lim membrane rau pom

Lim thiab tshuab hauv qab nitrogen rau kaum zaug ua ntej ntxiv 1ml ntawm chloroform mus rau qhov ntim tas li rau GC / MS tsom xam.

2 Kev tshwm sim

Rotenone tau kuaj pom los ntawm cov qauv, thiab cov txiaj ntsig tau pom hauv daim duab 1 txog daim duab 3.

8 Kev Tsim Nyog

Tom qab xyoo ntawm kev tsim khoom, peb lub tuam txhab tau txais daim ntawv tso cai tsim khoom tsim nyog.

9 Tus khub

Peb muaj ntau tus neeg koom tes mus sij hawm ntev thoob plaws ntiaj teb.

10 Cov neeg siv khoom xyuas

Peb tau txais kev qhuas ntau ntawm lwm lub platform, thiab peb cov neeg siv khoom tau zoo siab heev ntawm cov khoom tsim los ntawm peb lub tuam txhab.

11 Exhibition

Peb lub tuam txhab koom nrog ntau yam kev nthuav qhia hauv tsev thiab txawv teb chaws txhua xyoo, thiab sib txuas lus nrog cov neeg siv khoom ntsej muag.

Cim npe nrov: rotenone hmoov, manufacturers, lwm tus neeg, Hoobkas, lag luam wholesale, yuav, nqe, zoo tshaj plaws, tej, kev muag khoom